Technical Grade Liquid Acid Protease AP-100
Halal and Kosher Certified
An ideal protease for ethanol industry, suitable for high concentration fermentation, promotes yeast growth and enables
higher ethanol yields.
Acid Protease AP-100 is made from Aspergillus niger using submerged fermentation, extraction, and refining techniques.
AP-100 can effectively hydrolyze proteins under acidic conditions.
AP-100 can be used in the production of fuel ethanol where protein
hydrolysis is needed under low pH conditions.
Declared Enzyme: Acid Protease
Systematic Name: EC 184.108.40.206
Activity: 100,000U/ml (minimum)
Appearance: Light brown Liquid
Product pH: 3.5 to 6.0
Specific gravity: 1.15 to 1.25 g/ml
EFFECT OF pH
The effective pH range of AP-100 is 2.0 to 6.0, with an optimum pH
from 2.5 to 4.0.
EFFECT OF TEMPERATURE
The activity of AP-100 is effective in the range from 30°C to 65°C,
with an optimum performance obtained at 30°C to 50°C. The exact
temperature optimum depends on actual process conditions including
time, pH, and substrate nature and substrate concentration.
EFFECT OF METAL IONS
AP-100 is activated by Mn2+, Ca2+ and Mg2+ ions. Heavy metal ions
such as Cu2+, Hg2+ and Al3+ will inhibit the enzyme activity of
ADVANTAGES AND PERFORMANCE BENEFITS
Use of AP-100 in fermentation process offers the following
- Improved fermentation ,saccharification and oil extraction
AP-100 can hydrolyze the proteins present in the raw materials,
destroy the interstitial cell wall structure of starch granules,
thereby release the starch wrapped in the granules, result in
improved saccharification as well as free bound oil.
Free Amino Nitrogen (FAN) is the most effective nitrogen source for
yeast. The addition of AP-100 during alcoholic fermentation can
effectively hydrolyze proteins and peptides in the raw materials so
as to generate FAN that the yeast can absorb. The absorption of the
free amino nitrogen promotes yeast growth, increases the quantity
of yeast in the fermented mash, and shortens the fermentation
The use of AP-100 in the propagation and/or fermentation steps to
form Free Amino Nitrogen (FAN) can not only shorten the
fermentation cycle but also increase the output of alcohol, improve
ethanol tolerance and survival rate of yeast.
- Reduces urea/ammonia usage
The increased FAN produced by use of AP-100 may result in a reduced
need for urea/ammonia addition. Exact reduction levels will need to
be determined based upon fermentation results.
In intermittent fermentation, the entire dose of AP-100 should be
added all at once after the fermentation tank is filled
approximately 30%. In continuous fermentation, AP-100 should be
added continually, with the dosage rate dependent on the amount of
mash fed at the inlet of the fermentation tank.
|Package:||25kg, 30kg plastic drums or 1125kg IBC totes|
|Shelf-life and Storage:|
Typical shelf-life is six months if this product is stored at 0∼
10°C in its original packaging, sealed and unopened, protected from
the sunlight. Prolonged storage and/or adverse conditions such as
higher temperatures may lead to a higher dosage requirement, thus
should be avoided.