Acid Protease AP-150
Technical Grade Liquid Protease for Ethanol
Halal and Kosher Certified
|Declared Enzyme||Acid Protease|
|Systematic Name||EC 220.127.116.11|
|Activity||150,000 U/ml (minimum)|
|Appearance||Light to dark brown Liquid|
Acid Protease AP-150 is produced from selected strains of Aspergillusniger through submerged fermentation and extraction and refining
processes. It can catalyze the hydrolysis of proteins under acidic
AP-150 is applicable to hydrolysis of proteins with acidic medium.
EFFECT OF PH, TEMPERATURE AND IONS
Effective pH 2.0 to 6.0
Optimal pH 2.5 to 3.5
Effective temperature 30 to 65°C
Optimal temperature 40 to 50°C
Activated by Mn2+, Ca2+, Mg2+
Inhibitors: Cu2+, Hg2+, Al3+
ADVANGTAGES AND BENEFITS
A robust protease suitable for high concentration fermentation in
ethanol production, capable to promote yeast growth and enable more
ethanol yields in a cost-effective way.
- Promote raw material particles dissolved
AP-150 can hydrolyze the proteins of the raw materials, thus
destroy the interstitial cell wall structure of starch granules, so
as to release the starch wrapped in the granules,
which is conducive to saccharification.
- Promote the growth of yeast with reproduction
Free amino nitrogen (FAN) is the most effective nitrogen source for
yeast. Adding AP-150 during alcoholic fermentation can effectively
hydrolyze proteins and peptides in the
raw materials so as to generate FAN that yeasts can absorb, thus
promote the growth of yeasts, increase the quantities of yeasts in
the fermented mash, and shorten fermentation cycle.
- Beneficial to high concentration fermentation
In high concentration fermentation of alcohol, it is mainly
nutritional deficiencies rather than the accumulation of alcohol
that leads to the decrease of fermentation activity.
Free amino acids (FAN) can not only shorten the fermentation cycle
but also increase the output of alcohol, improve ethanol tolerance
and survival rate of yeasts.
Therefore, AP-150 is the best nutrient for yeast growth and
accelerator for fermentation.
In intermittent fermentation, it should be added at one time when
the fermentation tank is filled with one third materials. In
continuous fermentation, it should be added consecutively depending
on the amount of saccharification mash fed at the inlet of
Recommended dosage of AP-150 is 0.001 to 0.01% w/w (dry solids
basis). Actual dosage should be depending on the raw materials and
process of production.
PACKAGING, SHELF-LIFE, STORAGE AND SAFETY
AP-150 is packaged in 25kg, 30kg plastic drums or 1125kg IBC totes.
Customized packaging is available upon request.
AP-150 has been formulated for optimal stability. Typical
shelf-life is six months if it is stored below 25°C in its original
packaging, sealed and unopened, protected from the sunlight. Its
shelf-life will be twelve months if it is stored properly at
0～10°C. Prolonged storage and/or adverse conditions such as higher
temperatures may lead to a higher dosage requirement, thus should
Inhalation of enzyme dust and mists should be avoided. In case of
contact with the skin or eyes, promptly rinse with water for at
least 15 minutes. For detailed handling information, please refer
to the Safety Data Sheet.